Open AccessCharacterization of cCe13, a Member of the Pepper Endo‐β‐1, 4‐Glucanase Multigene Family
Author(s) -
Trainotti Livio,
Ferrarese Luca,
Casadoro Giorgio
Publication year - 1998
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1998.00121.x
Subject(s) - abscission , glucanase , biology , pepper , gene family , complementary dna , genomic dna , gene , botany , horticulture , genetics , gene expression
In pepper plants the enzyme endo‐β‐1,4‐glucanase (EGase) is encoded by a multigene family. Here is described the characterization of cCe13, a cDNA which codes for the third EGase known so far in this plant. The known members of this family are present as single copy genes, as demonstrated by a Southern analysis of the genomic DNA. Analysis of the expression of cCe13 demonstrates that the highest levels of the cCe13 mRNA are found in abscission zones of leaves and flowers activated by treatments with the plant hormone ethylene. However, the amounts of the cCe13 transcripts (detectable only by RT‐PCR) are always much lower than those of cCe12 so that the latter can be regarded as the “abscission” EGase while cCe13 is likely to perform a role ancillary to that of cCe12.