Open AccessSpontaneous rate of sister chromatid exchanges (SCEs) and BrdU dose‐response relationships in mitotic chromosomes of goat ( Capra hircus L.)
Author(s) -
Berardino Dino,
Jovino Valeria,
Lioi Maria Brigida,
Scarfi Maria Rosaria,
Burguete Isabel
Publication year - 1996
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1996.t01-1-00137.x
Subject(s) - sister chromatids , microgram , sister chromatid exchange , biology , bromodeoxyuridine , mitosis , chromatid , capra hircus , microbiology and biotechnology , chromosome , ploidy , andrology , genetics , cell growth , dna , zoology , in vitro , medicine , gene
The spontaneous level of sister chromatid exchange (SCEs) in the goat, estimated by exposing peripheral blood lymphocytes to 0.1 μg/ml of 5‐bromodeoxyuridine (BrdU), was 3.28 ± 1.71 SCE/cell, 1.64 SCE/cell generation and 0.027 SCE/chromosome. The dose–response curve of SCE/cell, observed by exposing the cells to 0.1, 0.25, 0.5, 1.0, 2.5, and 5.0 μg/ml of BrdU, rose rapidly from 0.1 to 0.5 μg/ml, remained fairly stable from 0.5 to 1.0 μg/ml and rose less rapidly from 1.0 to 5.0 μg/ml of BrdU. The frequency distribution of sister chromatid exchanges/cell and that of chromosomes showing various number of exchanges followed the Poisson probability at all BrdU levels; only at 5.0 μg/ml of BrdU was the fit found on the border of the 5% probability level. The usefulness of determining the spontaneous level of SCE/cell in domestic animals is discussed in relation to its possible application for a more precise evaluation of the genotoxic effects of environmental pollutants.