Open AccessAg‐NOR Staining and Satellite Association in Bone Marrow Cells from Patients with Mycosis Fungoides
Author(s) -
Pedrazzini Estela,
Slavutsky Irma
Publication year - 1995
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1995.00009.x
Subject(s) - biology , mycosis fungoides , staining , nucleolus organizer region , bone marrow , metaphase , nucleolus , pathology , satellite , immunology , microbiology and biotechnology , chromosome , genetics , lymphoma , medicine , cytoplasm , gene , engineering , aerospace engineering
Silver staining of nucleolus organizing regions (Ag‐NORs) of acrocentric chromosomes and the frequency of satellite association (SA) in bone marrow (BM) cells from 7 patients with mycosis fungoides (MF), were studied. BM samples of 7 normal healthy individuals were taken as controls. The mean number of Ag‐NORs per metaphase was increased in patients (7.20 ± 0.25) compared with controls (5.40 ± 0.16) (p < 0.002), related with the increase of the D group. Moreover, a significant higher percentage of Ag‐NOR positive cells in patients (71.7 ± 3.9) than controls (48.0 ± 7.8) (p < 0.02), was seen. The analysis of SA revealed a significant increase in the percentage of cells with 1–2 association pairs (ASP s ) in patients with respect to their controls (p < 0.05), and a trend to a decrease in the percentage of cells without ASPs. Furthermore, a correlation between the number of Ag‐NORs and the mean of ASPs per cell was also found for patients (r k = 0.65; p < 0.05). These results may be associated with a certain degree of immaturity, a high proliferative activity and modifications of the growth rate of BM cells in MF patients.