Open AccessA New Method for Differential Staining of Brassica Metaphase Chromosomes, and Karyotypes of B. Campestris, B. Oleracea, and B. Napus
Author(s) -
OlinFatih Majlis
Publication year - 1994
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1994.00253.x
Subject(s) - biology , brassica , karyotype , brassica oleracea , differential staining , mitosis , metaphase , staining , chromosome , botany , heterochromatin , genetics , gene
Mitotic cells from three Brassica species, B. campestris L., B. oleracea L., and B. napus L. have been differentially stained after cold treatment and application of the ASG method for G‐banding. Karyotypes and idiograms are presented. In all three species the chromosomes can be matched to individual pairs. The heterochromatic satellite next to the nucleolar organizer region (NOR) differs from the rest of the chromosomal material by having a very faint colour. It responds to the staining procedure in the same way as cold sensitive regions of chromatin described in other species. In Brassica this type of reaction is apparently limited to the satellite next to the active NOR.