Open AccessReplication Patterns of Sex Chromosomes in Melandrium Album Female Cells
Author(s) -
Široký Jiří,
Janoušek Bohuslav,
Mouras Armand,
Vyskot Boris
Publication year - 1994
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1994.00175.x
Subject(s) - biology , heterogametic sex , chromosome , genetics , x chromosome , mitosis , replication timing , dna replication , microbiology and biotechnology , dna , gene
Autoradiographic analysis of chromosome replication patterns in heterogametic male and homogametic female hairy‐root cell lines of a dioecious plant, Melandrium album , has shown an early replication of the only X and a conspicuous difference between the two X chromosomes, respectively. This divergence could be significantly reduced by a 5‐azacytidine treatment, thus confirming our recently published results on the different degree of DNA methylation of the X chromosomes in female cells. The early replicating and aberration prone X chromosome seems to be less condensed, too. [ 3 H]thymidine labelling patterns as well as [ 3 H]uridine induced chromosome aberrations do not indicate any differences between the long and short arms of the X chromosomes. The presented data support the hypothesis that one of the two X chromosomes in M. album female cells could be inactivated and hypermethylated as described in mammals.