Open AccessEvolution of Hordein Gene Organization in Three Hordeum Species
Author(s) -
Pelger Susanne,
Säll Torbjörn,
Bengtsson Bengt O.
Publication year - 1993
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1993.00219.x
Subject(s) - hordein , biology , hordeum , genetics , hordeum vulgare , chromosome , aegilops , gene , b chromosome , botany , triticeae , storage protein , ploidy , karyotype , poaceae , genome
The inheritance pattern of hordein, the seed storage protein in barley, has been studied in two wild Hordeum species, H. murinum and H. pusillum. Three different diploid populations of each species were crossed, and the F 1 plants were self‐pollinated. The seeds with an F 2 genotype were studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE). Several differences could be observed in the banding patterns of the parental populations. The analysis of recombinant banding patterns showed that in H. murinum there are at least four segregating loci, of which two code for B‐ and two for C‐hordein. All the loci are linked on the same chromosome. In H. pusillum at least six segregating loci were found, of which three code for B and three for C‐hordein. Five of the loci are linked, while the sixth showed independent segregation. The organization of the hordein genes differs not only between these two species but also between them and the different forms of H. vulgare , as well as with other species belonging to the tribe Triticeae. Extensive rearrangements must obviously have taken place among the members of the hordein gene family since the divergence of the species in the Hordeum genus. The possibility is discussed that the genes have been moved through transposition, a possible mechanism for the physical divergence of tandemly repeated sequences.