Open AccessMicronucleus Induction in Human B and T Lymphocytes Separated by an Immunomagnetic Technique
Author(s) -
Högstedt Benkt,
Karlsson Anita,
Bratt Ingrid,
Holmén Anders
Publication year - 1993
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1993.00099.x
Subject(s) - mitomycin c , micronucleus test , incubation , biology , micronucleus , microbiology and biotechnology , cytotoxic t cell , incubation period , cytotoxicity , immunology , in vitro , biochemistry , toxicity , genetics , medicine
Micronuclei were induced in human lymphocytes by mitomycin C or gamma radiation. After the incubation the cells were separated in B‐ and T‐cells by an immunomagnetic method, i.e., Dynabeads. The cultures were stimulated by PHA. The effect of different incubation times (60–110 h) as well as different doses of mitomycin C (0–100 nmol/l) and gamma radiation (0–2 Gy) at an incubation time of 72 h were studied. There were significantly higher frequencies of micronuclei in B‐ than in T‐cells, when micronuclei were induced by mitomycin C. This was not found with gamma radiation, probably because of a cytotoxic effect of this agent on the B‐cells.