Open AccessAg‐NOR staining and satellite association in lymphoproliferative disorders
Author(s) -
PEDRAZZINI ESTELA,
SLAVUTSKY IRMA
Publication year - 1992
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1992.tb00563.x
Subject(s) - biology , chronic lymphocytic leukemia , lymphoproliferative disorders , metaphase , nucleolus organizer region , mycosis fungoides , immunology , b cell , leukemia , pathology , microbiology and biotechnology , lymphoma , genetics , chromosome , medicine , antibody , gene
The nucleolar organizer regions (NORs) activity and the frequency of satellite associations (SA) in peripheral blood lymphocytes from patients with two chronic lymphoproliferative disorders were studied: 10 cases with B‐cell chronic lymphocytic leukemia (B‐CLL) and 10 with mycosis fungoides (MF). Thirteen healthy individuals formed the MF control group, and the oldest 7 constituted the B‐CLL control group. The mean of Ag‐NORs per metaphase was increased in B‐CLL patients (8.80 ± 0.63) compared with their controls (7.99 ± 0.90) (P < 0.025), meanwhile MF patients' value did not differ from their controls. In both disorders, the frequency of Ag‐NORs in the G chromosomes was increased. The analysis of SA in B‐CLL patients only revealed an increase in the frequency of cells with more than 4 ASPs (association pairs). Meanwhile, a significant higher mean of ASPs per cell in MF patients (1.74 ± 0.41) compared to controls (1.40 ± 0.24) (P < 0.05) was observed. Furthermore, a close correlation between cells with complexes of 3 or more chromosomes and the mean of ASPs per cell was also found in MF. In conclusion, an increase of the Ag‐NORs expression in B‐CLL patients and a modification in the degree of SA in MF patients were found.