Open AccessRepeated DNA sequences of Aegilops markgrafii (Greuter) Hammer var. markgrafii‘. Cloning, sequencing and analysis of distribution in Poaceae species
Author(s) -
BALDAUF F.,
SCHUBERT V.,
METZLAFF M.
Publication year - 1992
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1992.tb00207.x
Subject(s) - aegilops , biology , genome , poaceae , genetics , repeated sequence , dna sequencing , cloning (programming) , dna , botany , gene , computer science , programming language
Total DNA of Aegilops markgrafii (Greuter) Hammer var. markgrafii was shot gun cloned. From all the recombinants containing repetitive sequences 1–2% hybridized preferentially with the Ae. markgrafii genome and were almost absent in wheat. The cloned sequences are disperse distributed over the Aegilops chromosomes and show the typical features of eukaryotic repetitive DNA. Five specific probes were tested for their applicability in a screening program on 68 Poaceae accessions.