Open AccessPurification and characterization of a protein associated with genetically‐determined longevity difference in Dvosophila melanogaster
Author(s) -
OKANO AKIRA,
YONEMURA ISAMU,
SHIMIZU YOSHIHARU,
YANAGIDAIRA YASUNORI,
HASEKURA HAYATO,
BOETTCHER BARRY
Publication year - 1992
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1992.tb00022.x
Subject(s) - biology , longevity , drosophila melanogaster , melanogaster , genetics , gene
Earlier studies have shown a correlation between the presence of a 77 kDa protein in the proteins extracted from young adult Drosophila melanogaster (D.m.) and the autosomal longevity allele. A2 at the JmA locus. In this study, a 77 kDa protein has been isolated from pupae of D.m. of a long‐lived strain of genotype A 2A 2, and was purified by DEAE chromatography, Con A column chromatography, and two cycles of gel filtration. The purified protein has a molecular weight of 76,600 (by SDS‐PAGE), an isoelectric point of pH 6.5, and molar extinction coefficient A(1%/280) = 18.3. It is a glycoprotein containing 3.3 % hexose. Supplementing the food of D.m. with the purified protein at 5 × 10 ‐4 μg/ml, beginning at day 5 after emergence, resulted in an increase in the survival rate and maximal life span of both short‐lived and long‐lived strains of D.m.