Open AccessGenetic variation in ornamental apple trees and their seedlings (Malus, Rosaceae) revealed by DNA ‘fingerprinting’ with the M13 repeat probe
Author(s) -
NYBOM HILDE
Publication year - 1990
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1990.tb00694.x
Subject(s) - biology , minisatellite , dna profiling , ornamental plant , seedling , malus , rosaceae , genetic diversity , botany , restriction fragment length polymorphism , restriction enzyme , genetic marker , genetics , microsatellite , dna , polymerase chain reaction , population , allele , demography , sociology , gene
DNA samples from 23 ornamental apple trees as well as from 55 seedling offspring were separately digested with restriction enzymes and Southern blot hybridized to the minisatellite M13 repeat probe. The ripple trees yielded 8 or 10 different fragment patterns depending on the enzyme utilized whereas each seedling exhibited a unique pattern. Genetic diversity, as estimated by applying a similarity index to the fragment patterns to be associated with known levels of genetic relatedness, Paternity could be tentatively suggested for 21 of the seedlings investigated.