Open AccessLocalization of the glucose phosphate isomerase gene to the p12→q21 segment of chromosome 6 in pig by in situ hybridization
Author(s) -
CHOWDHARY B. P.,
HARBITZ I.,
MAKINEN A.,
DAVIES W.,
GUSTAVSSON I.
Publication year - 1989
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1989.tb00378.x
Subject(s) - biology , locus (genetics) , in situ hybridization , microbiology and biotechnology , gene , fluorescence in situ hybridization , gene mapping , chromosome , in situ , genetics , gene expression , physics , meteorology
The glucose phosphate isomerase (GPI) locus is closely linked to the halothane sensitivity locus in pig. The chromosomal localization of GPI in pig was confirmed to 6p12→q21 by using in situ hybridization. Of the total grains, 25 percent were located on chromosome 6, with about 65 percent clustered in the cent→q21 segment, suggesting the presence of the GPI gene on the very proximal part of the q arm. The efficiency of hybridization was found to be affected by ultraviolet irradiation of metaphases for RBA‐banding before hybridization. The irradiated metaphases had higher background grains than the non‐irradiated metaphases.