Open AccessLymphatic leukaemia cell line 3447 from the dog ‐A karyotypic analysis
Author(s) -
WELLING JUKKA,
STRANDSTROM HELENA,
KNUUTILA SAKARI
Publication year - 1988
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1988.tb00354.x
Subject(s) - biology , chromosomal translocation , karyotype , chromosome , mitosis , lymphocyte , genetics , cell , microbiology and biotechnology , immunology , gene
A continuous cell line was established from a dog with leukaemia. The ceils are considered T‐lymphocytic in origin, because they did not produce immunoglobulins, did not phagocytise, but did agglutinate with con‐canavalin A, phytohaernagglutinin, and pokewecd mitogen, and showed reactivity only in the lymphocyte acid phosphatase test. Their ability to grow on semisolid agar as well as the clinical findings confirm that they are malignant. The cells showed variability in chromosome number and extensive formation of Robertsonian translocations. Eivery cell is unique in chromosomal constitution; in addition there is extensive nullisomy involving many chromosomes and polysomy involving the smallest chromosomes in each cell.