Open AccessAdmixture of intact or lysed platelets to lymphocyte cultures results in higher chromosome aberration frequencies
Author(s) -
TOOR MOHAMMED A.,
HEIM SVERRE,
MANDAHL NILS,
HÖGSTEDF BENKT,
MITELMAN FELIX
Publication year - 1988
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1988.tb00304.x
Subject(s) - platelet , biology , lysis , in vitro , lymphocyte , breakage , chromosome , chromosome aberration , microbiology and biotechnology , dna , immunology , genetics , materials science , gene , composite material
We have examined the effect on chromosome aberration frequency of in vitro admixture of intact platelets or platelet lysates to lymphocyte cultures. A roughly linear correlation was found between platelet concentration in the culture medium and the level of breaks and gaps, with higher platelet concentrations corresponding to higher aberration frequencies. We draw the conclusion that a platelet‐derived factor interferes with the DNA breakage‐repair mechanism of dividing lymphocytes. Awareness of the importance of in vitro platelet concentrations is necessary in cytogenetic studies evaluating genotoxic effects of chemical substances or constitutionally determined breakage‐repair differences.