Protein loci in diploid tissue of Norway spruce ( Piceu abies  K.): description and interpretation of electrophoretic variability patterns | Zendy

Lagercrantz Ulf | Zendy; Ryman Nils | Zendy; Ståhl Gunnar | Zendy

Open Access

Protein loci in diploid tissue of Norway spruce ( Piceu abies K.): description and interpretation of electrophoretic variability patterns

Author(s) -

Lagercrantz Ulf,

Ryman Nils,

Ståhl Gunnar

Publication year - 1988

Publication title -

hereditas

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.819

H-Index - 50

eISSN - 1601-5223

pISSN - 0018-0661

DOI - 10.1111/j.1601-5223.1988.tb00295.x

Subject(s) - biology , ploidy , population , picea abies , endosperm , allele , population genetics , genetics , botany , evolutionary biology , gene , demography , sociology

An increasing interest has focused on the patterns for distribution of genetic vaniation in coniferous species, but difficulties in using diploid tissue for electrophoretic analyses have placed obvious limitations on the scale of studies aimed at delineating the genetic population structure of conifers. We describe electrophoretic procedures for analysis of enzyme loci in diploid tissue of Norway spruce ( Picea abies K.). Using bud extracts, a total of 25 loci (coding for 12 enzymes) considered usable in population surveys have been identified. All these loci are also expressed in endosperm tissue, but only 15 of them can be scored in needle extracts. The electrophoretic expressions of a total of 65 alleles observed after scoring some 5000 trees from most of the species' European distribution range are also described.

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