Open AccessIn situ induction of G‐bands and macrocoils in plant chromosomes
Author(s) -
XIAOFENG YANG,
ZILI ZHANG
Publication year - 1988
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1988.tb00180.x
Subject(s) - biology , secale , chromosome , homologous chromosome , hordeum vulgare , electron microscope , in situ , b chromosome , botany , genetics , karyotype , microbiology and biotechnology , poaceae , gene , optics , physics , meteorology
Four different methods including trypsin, urea, SDS and NaOH are presented for the in situ induction of G‐bands and macrocoils on the chromosomes of Secale cereale, Hordeum vulgare, and Viciafaba. The bands obtained were numerous and distributed along the whole length of the chromosomes, the number of the G‐bands was much interrelated with the condensation of chromosomes. The bands of homologous chromosomes in some cells were matchable. When the incubation time was beyond critical period for G‐banding, macrocoils were often revealed. Gyre number changed with chromosome contracting, and the direction of coils has showed different patterns. Transformation of G‐bands into macrocoils was observed in rye chromosomes. Some chromosomes showing G‐bands under light microscopy revealed spiral patterns under scanning electron microscopy. In this paper, some factors affecting G‐banding and the relationship between G‐bands and macrocoils in plant chromosomes were also primarily discussed.