Open AccessInfluence of serum concentration on chromosome breakage frequency
Author(s) -
JOHANSSON BERTIL,
MERTENS FREDRIK
Publication year - 1986
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1986.tb00523.x
Subject(s) - biology , chromosome , microbiology and biotechnology , breakage , significant difference , serum concentration , genetics , endocrinology , medicine , gene , materials science , composite material
Lymphocytes from four healthy individuals were cultured for 72 h in medium with 5, 10, 20 and 30% human and bovine serum, respectively. Half of the samples were exposed to N‐acetoxy‐2‐acethylaminofluorene (NA‐AAF) for 1 h. Chromosome preparations were scored with regard to frequency of structural chromosome aberrations, including gaps, breaks and exchanges. The total aberration frequencies were significantly higher in serum concentrations of 20 and 30% compared with 10%. No difference was observed between human and bovine serum.