Open AccessFlow cytometric karyotyping of mammals, using blood lymphocytes: Detection and analysis of chromosomal abnormalities
Author(s) -
MATSSON P.,
ANNERÉN G.,
GUSTAVSSON I.
Publication year - 1986
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1986.tb00516.x
Subject(s) - karyotype , biology , colcemid , metaphase , chromosome , flow cytometry , chromosomal analysis , microbiology and biotechnology , genetics , pathology , cell , medicine , gene
Flow cytometric karyotyping of two different species of mammals, with use of peripheral blood lymphocytes, was carried out. Human and porcine short‐term lymphocyte cultures were treated with colcemid prior to preparation of chromosomes in suspension. Different types of chromosomal abnormalities, human (47,+21) and porcine (38,t(13q‐;14q+)), were examined. Result of flow cytometric karyotyping of chromosomes in suspension were compared with G‐and Q‐banded karyotypes of metaphase chromosomes. The observed flow cytometric resolution for chromosome peaks ranged from 2.5 to 5.0% (coefficient of variation) when acceptable flow karyotypes were obtained. Flow karyotyping can be performed successfully in many cases, but cell growth conditions, preparation techniques and instrumentation are all sensitive factors that require consideration to achieve high quality results. These problems have to be solved before the method of flow karyotyping can be used for reproducible and routine diagnosis of chromosomal abnormalities.