Open AccessA proposed order of increasing divergence among five indispensable polypeptides in Bacillus
Author(s) -
DEGGERDAL A.,
GUNDERSEN W. B.
Publication year - 1985
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1985.tb00478.x
Subject(s) - rpob , biology , genetics , rna polymerase , bacillus subtilis , gene , rna , 16s ribosomal rna , bacteria
A strain of Bacillus subtilis, Mu8u5ul, has been used as the recipient in genetic transformation by DNA isolated from two different Bacillus licheniformis strains. Among the markers scored were five antibiotic resistance markers, all located to indispensable genes coding for RNA polymerase subunits, ribosomal and ribosome‐associated proteins. Our data indicate the following order of increasing evolutionary divergence: tuf rpsL [rpoB rpoC] efg. The data were not conclusive as to the internal ranking of the RNA polymerase genes, rpoB and rpoC. A sixth antibiotic resistance marker, Lpm R , formerly mapped in the rpoB, rpoC region, was included in the study. Our data did not give indication as to whether Lpm R is part of the rpoB or the rpoC gene.