Open AccessTransfer of methotrexate resistance by somatic cell hybridization
Author(s) -
JAKOBSSON ANNHELENE,
DAHLLOF BJÖRN,
MARTINSSON TOMMY,
LEVAN GÖRAN
Publication year - 1983
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1983.tb00901.x
Subject(s) - biology , dihydrofolate reductase , chinese hamster , microbiology and biotechnology , hybrid , gene , somatic fusion , somatic cell , cell culture , cell fusion , cell , genetics , botany
SEWATC13 mouse cells, resistant to 10 fig methotrexate (MTX)/ml, were fused to sensitive V79 Chinese hamster cells. Several resistant cell hybrids were isolated. In the mouse parental line MTX resistance is due to amplification of the dihydrofolate reductase gene, which cytologically is expressed in about 40 % of the cells as an HSR marker and in about 60 % as numerous DM. No DM were seen in 6 hybrids studied. Two hybrids analyzed in detail exhibited presence of the HSR marker in a majority of the cells. When subjected to 2‐dimensional gel electrophoresis, both hybrids displayed a protein spot at the location of mouse DHFR. The results indicate (1) the MTX resistance due to amplification of the DHFR gene can be transferred with cell fusion, and (2) that the transfer of an HSR marker appears to be preferred to transfer of DM in the cell system employed.