Open AccessChromosome banding by in vitro exposure to dA‐dT probes and BUdR. Relationships between DNA base clusters, replication pattern, and banding
Author(s) -
RØNNE MOGENS,
THUST RUDOLF
Publication year - 1983
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1983.tb00896.x
Subject(s) - biology , chinese hamster , g banding , giemsa stain , microbiology and biotechnology , mitosis , netropsin , chromosome , in vitro , dna , karyotype , dna replication , genetics , minor groove , gene
In vitro exposure of mammalian cells to dA‐dT probes partially inhibits chromosome contraction in mitosis. Especially dA‐dT rich regions are affected. Combined with BUdR incorporation in late S‐phase this treatment induces banding which depends on both replication pattern and location of dA‐dT rich regions. This method was used in normal mouse fibroblasts and in Chinese hamster V79‐E cells, with netropsin as the dA‐dT specific agent. Banding was observed both after conventional Giemsa and after FPG‐staining. In those cases where the G bands in general are dA‐dT rich and late replicating, the banding obtained with this technique corresponds to R‐bands.