Open AccessSimultaneous R‐banding and localization of dA‐dT clusters in human chromosomes
Author(s) -
RØNNE MOGENS
Publication year - 1983
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1983.tb00601.x
Subject(s) - biology , giemsa stain , metaphase , staining , karyotype , in vitro , chromosome , microbiology and biotechnology , g banding , genetics , gene
In vitro exposure of different cell lines to dA‐dT probes is known to induce species‐specific modifications in metaphase chromosomes. In human lymphocytes the main measurable effect is a general inhibition of chromosome contraction. The inhibition is pronounced in specific regions of the human karyotype, so these regions appear elongated. Simultaneous in vitro exposure of human PHA‐stimulated lymphocytes to BUdR and one of the dA‐dT specific agents distamycin A or Hoechst 33258 for 6.5 h prior to harvest induces R‐banded chromosomes both after conventional Giemsa and FPG‐staining. Because of the banding the position of the most affected regions can be precisely determined. Thus the combined in vitro exposure to BUdR and one of the dA‐dT specific agents offers a simple and precise means to localize major dA‐dT clusters in mammalian genomes with traditional Giemsa staining, and provides after FPG‐staining high resolution R‐bands.