Open AccessLocalization of SCEs and their possible relationship to dA dT‐ or dGdC‐clusters, respectively, in Chinese hamster V79‐E chromosomes
Author(s) -
THUST RUDOLF,
RØNNE MOGENS
Publication year - 1982
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1982.tb00862.x
Subject(s) - sister chromatids , netropsin , biology , chinese hamster , chromatid , metaphase , chromosome , genetics , elongation , microbiology and biotechnology , dna , biophysics , materials science , ultimate tensile strength , metallurgy , minor groove , gene
The localization of SCEs was studied by simultaneous treatment of V79‐E cells with netropsin‐HCl and BUdR. The SCEs occurred in a non‐random pattern, but no clear‐cut correlation of SCEs and C‐band heterochromatin or dAdT‐clusters, resp., was observed. BUdR substitution completely abolishes the differential elongation pattern of chromosomes obtained after in vitro treatment with netropsin alone, but does not prevent an overall chromosome elongation. Netropsin enforces the unequal stretching of sister chromatids produced by BUdR.