Open AccessPotentiation by hydroxyurea in G2‐prophase of thiotepa‐ and bleomycin‐induced chromosomal aberrations in Chinese hamster cells
Author(s) -
HANSSON KERSTIN,
HARTLEYASP BERYL
Publication year - 1981
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1981.tb01726.x
Subject(s) - chinese hamster , chromatid , prophase , biology , bleomycin , sister chromatids , genetics , thiotepa , mitosis , microbiology and biotechnology , chromosome , meiosis , dna , chemotherapy , gene , cyclophosphamide
The potentiating effect of 5times10 ‐3 M hydroxyurea in G2‐prophase on chemically induced chromosomal aberrations has been studied in Chinese hamster cells. Hydroxyurea, when given during the last 2 h before fixation, enhanced the frequency of aberrations induced by both S‐dependent (thiotepa) and S‐independent (bleomycin) agents. The most striking effect was seen on the frequencies of gaps and chromatid breaks but an increase was also found in the number of iso‐chromatid breaks of the non‐union type. The frequencies of exchanges and iso‐chromatid breaks of the sister‐union type were comparatively little affected. The results indicate that hydroxyurea inhibits a repair system in G2‐prophase involved in the formation of chromosomal aberrations in mammalian cells.