Open AccessEffects of parafluorophenylalanine on chromosome structure in human lymphoid cells and Chinese hamster V79‐E cells
Author(s) -
ANDERSEN OLE,
RØNNE MOGENS
Publication year - 1981
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1981.tb01324.x
Subject(s) - chinese hamster , biology , chromosome , metaphase , in vitro , hamster , genetics , cell culture , microbiology and biotechnology , gene
The effect of in vitro exposure of human lymphoid cells and Chinese hamster V79‐E cells to the phenylalanine‐analog parafluorophenylalanine under various experimental conditions has been studied. For both cell types, long‐time PFPA exposure combined with short‐time colchicin treatment results in a decrease in average chromosome length, compared with control cultures. The overcontraction of metaphase chromosomes from human lymphoid cells observed after long‐time colchicin‐exposure was to some degree counteracted by simultaneous long‐time parafluorophenylalanine exposure. The results are discussed in relation to the known biochemical and biological effects of parafluorophenylalanine.