Open AccessEffect of controlled colcemid exposure on human metaphase chromosome structure
Author(s) -
RØNNE MOGENS,
NIELSEN KIRSTEN VANG,
ERLANDSEN MOGENS
Publication year - 1979
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1979.tb01640.x
Subject(s) - colcemid , prometaphase , metaphase , biology , prophase , chromosome , microbiology and biotechnology , genetics , mitosis , meiosis , gene
When human lymphoid cells are exposed to colcemid overnight at 4°C and subsequently incubated at 37.5°–38°C, variation in incubation time offers a simple method to produce chromosome slides with a high frequency of either prophase, prometaphase, or metaphase chromosomes.