Open AccessIncreased sister chromatid exchange in megaloblastic anaemia — Studies on bone marrow cells and lymphocytes
Author(s) -
KNUUTILA SAKARI,
HELMINEN EILA,
VUOPIO PEKKA,
CHAPELLE ALBERT
Publication year - 1978
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1978.tb01273.x
Subject(s) - sister chromatids , biology , bone marrow , chromatid , micronucleus test , sister chromatid exchange , phytohaemagglutinin , erythroblast , centromere , lymphocyte , chromosome , megaloblastic anemia , genetics , microbiology and biotechnology , immunology , medicine , endocrinology , haematopoiesis , stem cell , vitamin b12 , dna , toxicity , gene
Sister chromatid exchanges (SCE) and chromosome aberrations were scored in bone marrow cells and phytohaemagglutinin‐stimulated lymphocytes of 8 patients with megaloblastic anaemia. Micronuclei were also studied from erythroblast/megaloblast precursors. Unlike the bone marrow cells of the controls, a proportion of the bone marrow cells of patients with megaloblastic anaemia showed evidence of 1) a highly increased (even 10‐fold) number of SCE (> 50 per cell), 2) notable chromosome aberrations (i.e. despiralization, centromere separation, chromatid exchanges, and “pulverization”), 3) an increased proportion of micronuclei (1% to 7% as compared to 0% to 0.4% in the controls). The lymphocytes of patients with megaloblastic anaemia also had significantly (P<0.001) higher SCE (mean 9.7) than the controls (mean 7.9), even though these cells had no notable chromosome aberrations.