Open AccessStructure of the chromosomes of Scotch pine
Author(s) -
KUPILAAHVENNIEMI SIRKKA,
HOHTOLA ANJA
Publication year - 1978
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1978.tb01261.x
Subject(s) - giemsa stain , biology , feulgen stain , centromere , chromatid , heterochromatin , g banding , genetics , evolutionary biology , karyotype , staining , chromosome , gene
Staining the chromosomes of Scotch pine with a simplified Giemsa procedure reveals a differentially stained heterochromatic pattern, on the basis of which four types of chromosomes can be recognized. Irregularities in the size of chromosomes as well as in the occurrence of secondary constrictions are observed. The chromatids do not separate before the centromeres divide and start to pull them apart. The use of Chlorazol black E in combination with the Giemsa procedure or with the Feulgen procedure brings out a more detailed banding pattern than by using the Giemsa procedure alone.