Open AccessThe chromosome complement of a measles‐carrier human cell line in comparison to the cell line of origin
Author(s) -
HENEEN WAHEEB K.
Publication year - 1976
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1976.tb01574.x
Subject(s) - biology , karyotype , hela , measles virus , chromosome , microbiology and biotechnology , cell culture , measles , cell type , cell , genetics , virology , gene , vaccination
G‐ and C‐banding techniques were used for a karyotype analysis of measles‐carrier human cells and the cell line of origin, Lu106, which is most likely a HeLa‐derivative. The carrier cells showed a wider distribution of chromosome numbers and a lower stemline number (64–66) than in Lu106 (70). The percentage of marker chromosomes was slightly but significantly higher in carrier cells (40.7%) than in Lu106 (35.7%). A certain proportion (41.8%) of the carrier markers were new types, the origin of which might be attributed to structural rearrangements in Lu106 normal or marker chromosomes. They could also represent Lu106 markers not recorded in the sample analyzed of these cells. Certain new marker types were possible to identify as modified Lu106 marker types whereas others were similar to certain HeLa marker types. The total relative length of the chromosomal complement in the carrier cells was slightly lower than in Lu106. These values were also more variable in the carrier cells. This was largely due to variations in the total lengths of the new marker types. Large markers were common, possibly indicating a frequent involvement of large chromosomes in rearrangements. Markers are apparently more prone to undergo further structural changes. The lower uniformity and stability of the carrier cells is most likely a consequence of their persistent infection with measles virus.