Open AccessFusion of human cells with carrot protoplasts induced by polyethylene glycol
Author(s) -
DUDITS D.,
RASKO I.,
HADLACZKY GY.,
LIMADEFARIA A.
Publication year - 1976
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1976.tb01545.x
Subject(s) - protoplast , hela , daucus carota , cell fusion , polyethylene glycol , heterokaryon , biology , interphase , fusion , staining , cell , microbiology and biotechnology , biochemistry , biophysics , botany , genetics , linguistics , philosophy , gene , mutant
HeLa cells were fused with protoplasts of carrot ( Daucus carota ) by the use of polyethylene glycol as fusion agent. The two types of nuclei can be recognized in the heterokaryon by their differential staining ability and by the tritium labelling of HeLa cells. Seventy‐two hours after fusion the cells appeared surrounded by cell walls and in some cases contained HeLa and carrot fused interphase nuclei. The percentage of fusion is estimated to be approximately 0.3 to 0.6%.