Open AccessBone‐marrow chromosomes in healthy subjects
Author(s) -
KNUUTILA SAKARI,
SIMELL OLLI,
LIPPONEN PIRJO,
SAARINEN IRJA
Publication year - 1976
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1976.tb01534.x
Subject(s) - chromatid , biology , metaphase , chromosome , aneuploidy , clone (java method) , polyploid , bone marrow , mitosis , karyotype , genetics , ploidy , microbiology and biotechnology , andrology , immunology , dna , gene , medicine
The frequency of aberrations in normal human bone‐marrow chromosomes was measured in 20 healthy subjects aged 24–37 years. The normal limits of indices for chromosome and chromatid aberrations, for aneuploidy and for polyploidy are extremely narrow in young healthy subjects. Of 2914 cells in which chromosomal aberration were studied such aberrations were seen in 0.4%. Most of them were chromatid breaks with the breakpoints randomly distributed. The percentage of hypodiploidy was 0–16.7. The number of hypodiploid mitoses correlated significantly with the number of broken metaphases. Moreover, because in all subjects the missing chromosome(s) was not consistently absent from every metaphase, hypodiploidy was probably a result of preparation technique. Hyperdiploidy was never observed. Among 29,062 dividing cells the frequency of polyploidy was 0.33%. Of these polyploid divisions 60% were tetraploid, 25% octoploid, 8% 16‐ploid and 7% polyploids of other multiples. In only one subject an abnormal clone was found. In this clone there was an extra marker and one G chromosome was missing.