Open AccessG‐banding in Rous rat sarcomas during serial transfer: Significant chromosome aberrations and incidence of stromal mitoses
Author(s) -
Levan Göran,
Mitelman Felix
Publication year - 1976
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1976.tb01190.x
Subject(s) - biology , rous sarcoma virus , chromosome , mitosis , karyotype , ploidy , sarcoma , population , stromal cell , virology , genetics , microbiology and biotechnology , pathology , virus , cancer research , medicine , demography , sociology , gene
Four subcutaneous rat sarcomas, originally induced by Rous sarcoma virus (RSV), had been submitted, as primary tumors, to chromosome analysis by conventional technique. They were recovered from the tumor bank, inoculated in vivo, and their chromosomes were again studied during serial passage, now with G‐banding. The 3 sequential steps of chromosome changes, earlier found to be characteristic of RSV tumors — additions of in turn 1 B, 1 C13 and 1 C12 chromosome — were found: all 3 steps in 1 tumor, the first 2 steps in 2 tumors and the first step in all 4 tumors. The B chromosome added in the first step had previously been determined to group only; it was now identified as a B7 chromosome. In the early passages of 3 of the transplanted tumors a sizable proportion of the tumor cell population consisted of normal diploid cells. Among them 45% had sex chromosomes of the sex opposite to that of the primary tumor and evidently corresponding to the sex of the incidental host. It is suggested that this situation is due to the induction by the transformed cells of mitoses in normal stromal cells, which thus take part in the growth of RSV tumors to an extent greater than usually realized.