Open AccessMeiosis and pollen formation in haploid Thuja plicata gracilis Oud
Author(s) -
SIMAK MILAN,
GUSTAFSSON ÅKE,
RAUTENBERG WIEBKE
Publication year - 1974
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1974.tb01341.x
Subject(s) - biology , strobilus , pollen , ploidy , thuja , botany , meiosis , microspore , cutting , gibberellin , horticulture , stamen , genetics , gene
Male strobili development and the course of microsporogenesis were studied on a tree growing in the Botanical Garden at Stockholm as well as on cuttings from a haploid and a diploid plant of Thuja plicata. The plants from cuttings were kept in a phytotron experiment at different photoperiods and treated with gibberellin in order to get rich flowering. The development of the strobili and the course of microsporogenesis were very variable under different ecological conditions. The best result (similar to that of the tree in Botanical Garden) was obtained when the plants from cuttings were kept for 17 weeks in long day during which period the plants were sprayed with gibberellin, twice a week. A rich initiation of buds was obtained but the development remained arrested at this initiation stage (corresponding to preprophase‐pachytene). After transferring the plants to short day the strobili development and microsporogenesis continued and were completed after 14 weeks. Thus, the photoperiodical sequence necessary for complete development of pollen grains was long day — short day. The pollen grains in diploid plants were normally developed but those in haploid ones showed irregularities caused by meiotic disturbances.