Open AccessCytogenetic investigations on an Ehrlich ascites tumor, and four sublines resistant to daunomycin, adriamycin, vincristine and vinblastine
Author(s) -
HASHOLT LIS,
DANØ KELD
Publication year - 1974
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1974.tb00942.x
Subject(s) - vinblastine , biology , vincristine , clone (java method) , giemsa stain , microbiology and biotechnology , neoplasm , ploidy , g banding , tumor cells , cancer research , chemotherapy , karyotype , genetics , gene , chromosome , cyclophosphamide
The karyotypic profiles of an Ehrlich ascites tumor and of four sublines of this tumor were analysed. The four sublines have been selected for their resistance to daunomycin, adriamycin, vincristine and vinblastine, respectively. All the sublines showed mutual cross resistance to the four agents. Some of the mitoses were stained with conventional Giemsa technique, whereas other mitoses were stained with Giemsa banding technique. The wild‐type tumor and the vincristine‐resistant tumor were found to be near‐tetraploid, whereas the three other sublines were hyper‐diploid. A number of marker chromosomes were found. It is concluded that the mutual cross resistance of the sublines is not due to selection of the same cell clone from the wild type tumor.