Open AccessAmplification of ribosomal DNA in Acheta
Author(s) -
JAWORSKA HALINA,
AVANZI SILVANA,
LIMADEFARIA A.
Publication year - 1973
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1973.tb01121.x
Subject(s) - cytoplasm , acheta , biology , dna , rna , microbiology and biotechnology , ribosomal rna , genetics , gene , ecology , cricket
In Acheta two types of nucleolar structures which contain DNA move as DNA‐RNA assemblies from the nucleus to the cytoplasm. This process takes place by intracytosis and a piece of the nuclear envelope follows these DNA‐RNA components into the cytoplasm where they reach the cell membrane. The ribosomal DNA and the ribosomal cistrons are localized in the first of these components and the second one is formed in close contact with this DNA. As a further test of the presence of DNA in these two types of structures before and after their migration to the cytoplasm the binding of H 3 ‐actinomycin D was studied. The two components bind actinomycin heavily, as shown by the presence of silver grains over these structures, both in the nucleus and the cytoplasm. The fact that the labelling is seen in the DNA after it has reached the cytoplasm indicates that this macromolecule has not been broken down immediately.