Open AccessFluorescence study of interphase nuclei and double Y chromosomes
Author(s) -
ÅKESSON HANS OLOF,
FORSSMAN HANS,
WAHLSTRÖM JAN
Publication year - 1971
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1971.tb02435.x
Subject(s) - interphase , biology , fluorescence , karyotype , chromosome , genetics , physics , optics , gene
A study was made of 118 institutionalized mentally handicapped men with behaviour disorders in order to screen the occurrence of double Y‐chromosomes. The screening was based on identifying the extra Y‐chromosomes in the interphase nuclei by fluorescence. Our intention was to test the sentisivity of this method in that respect. Altogether, we found 8 cases with cells having more than one brightly fluorescing area, and two of them proved by ordinary blood karyotyping to have double Y‐chromosomes. It is known that other areas than those in the Y‐chromosome may fluoresce. Thus by using interphase nuclei to determine the frequency of double Y‐chromosomes by fluorescence, a number of falsely positive results is given. Nevertheless, we have found the procedure extremely valuable for screening purposes.