Open AccessThe influence of culture method on the satellite association pattern in human lymphocytes: macroculture versus microculture
Author(s) -
HANSSON ALF
Publication year - 1970
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.1970.tb02332.x
Subject(s) - microculture , biology , table (database) , satellite , association (psychology) , genetics , cell culture , psychology , physics , astronomy , computer science , data mining , psychotherapist
The frequency and pattern of satellite association (sa) were studied in short‐term blood cultures from 7 normal persons. From each person, pairs of samples were analyzed after growth in macroculture and microculture, respectively. Consistent differences were found between the two series of samples: in relation to the macrocultures, the microcultures showed an increase in percentage of cells with one or more sa complexes (Table 1), in the number of sa complexes per cell (Table 2), and in the number of associating acrocentrics (Table 3 and 4). Most of these differences were statistically significant. The present results are opposite to those of Z ang and B ack (1968), obtained in similar experiments. The necessity of further elucidation of how methodological factors influence the study of sa is pointed out.