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The bactericidal effect of a Genius ® Nd:YAG laser
Author(s) -
Kranendonk AA,
Van Der Reijden WA,
Van Winkelhoff AJ,
Van Der Weijden GA
Publication year - 2010
Publication title -
international journal of dental hygiene
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.674
H-Index - 38
eISSN - 1601-5037
pISSN - 1601-5029
DOI - 10.1111/j.1601-5037.2009.00375.x
Subject(s) - aggregatibacter actinomycetemcomitans , tannerella forsythia , prevotella intermedia , fusobacterium nucleatum , porphyromonas gingivalis , medicine , microbiology and biotechnology , laser , agar plate , dentistry , periodontitis , bacteria , biology , optics , pathology , honeysuckle , physics , alternative medicine , genetics , traditional chinese medicine
Purpose: To evaluate the ‘in vitro’ bactericidal effect of the Nd:YAG laser (Genius, MØlsgaard Dental, Copenhagen, Denmark) on six periodontal pathogens. Methods: Suspensions of six different periodontal pathogens ( Aggregatibacter actinomycetemcomitans , Porphyromonas gingivalis , Prevotella intermedia , Tannerella forsythia , Fusobacterium nucleatum and Parvimonas micra ) were prepared in small Eppendorff tubes, and exposed to a Nd:YAG laser for five different periods of time. Laser settings used: Power 6 Watt (on a scale of 1–12 W), Frequency 50 Hz, Pulse duration 250 μs. After exposure to the laser, aliquots of the suspensions were spread on blood agar plates for bacterial counting. Results: After 5 s of laser exposure, there was a decrease in total colony forming units for all six selected microorganisms. After 15, 30 and 45 s, no viable bacterial cells could be retrieved. Conclusion: In this ‘in vitro’ model, 15 s of Nd:YAG laser use was found to be effective for total killing of the six tested periodontal pathogens.