Open AccessGenetic loss of D‐amino acid oxidase activity reverses schizophrenia‐like phenotypes in mice
Author(s) -
Labrie V.,
Wang W.,
Barger S. W.,
Baker G. B.,
Roder J. C.
Publication year - 2010
Publication title -
genes, brain and behavior
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.315
H-Index - 91
eISSN - 1601-183X
pISSN - 1601-1848
DOI - 10.1111/j.1601-183x.2009.00529.x
Subject(s) - nmda receptor , glycine , serine , schizophrenia (object oriented programming) , startle reaction , psychosis , startle response , agonist , psychology , endocrinology , chemistry , medicine , neuroscience , amino acid , receptor , biochemistry , biology , enzyme , psychiatry , reflex
Reduced function of the N ‐methyl‐ d ‐aspartate receptor (NMDAR) has been implicated in the pathophysiology of schizophrenia. The NMDAR contains a glycine binding site in its NR1 subunit that may be a useful target for the treatment of schizophrenia. In this study, we assessed the therapeutic potential of long‐term increases in the brain levels of the endogenous NMDAR glycine site agonist D‐serine, through the genetic inactivation of its catabolic enzyme D‐amino acid oxidase (DAO) in mice. The effects of eliminating DAO function were investigated in mice that display schizophrenia‐related behavioral deficits due to a mutation ( Grin 1 D481N ) in the NR1 subunit that results in a reduction in NMDAR glycine affinity. Grin 1 D481N mice show deficits in sociability, prolonged latent inhibition, enhanced startle reactivity and impaired spatial memory. The hypofunctional Dao 1 G181R mutation elevated brain levels of D‐serine, but alone it did not affect performance in the behavioral measures. Compared to animals with only the Grin 1 D481N mutation, mice with both the Dao1 G181R and Grin 1 D481N mutations displayed an improvement in social approach and spatial memory retention, as well as a reversal of abnormally persistent latent inhibition and a partial normalization of startle responses. Thus, an increased level of D‐serine resulting from decreased catalysis corrected the performance of mice with deficient NMDAR glycine site activation in behavioral tasks relevant to the negative and cognitive symptoms of schizophrenia. Diminished DAO activity and elevations in D‐serine may serve as an effective therapeutic intervention for the treatment of psychiatric symptoms.