Open AccessSelective breeding for catalepsy changes the distribution of microsatellite D13Mit76 alleles linked to the 5‐HT 1A serotonin receptor gene in mice
Author(s) -
Kondaurova E. M.,
Bazovkina D. V.,
Kulikov A. V.,
Popova N. K.
Publication year - 2006
Publication title -
genes, brain and behavior
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.315
H-Index - 91
eISSN - 1601-183X
pISSN - 1601-1848
DOI - 10.1111/j.1601-183x.2006.00212.x
Subject(s) - catalepsy , biology , allele , genetics , locus (genetics) , microsatellite , backcrossing , gene , endocrinology , dopamine , haloperidol
Catalepsy (pronounced motor inhibition) is a natural defensive reaction against predator. Recently, the quantitative trait locus for catalepsy was mapped on mouse chromosome 13 near the 5‐HT 1A serotonin receptor gene. Here, the linkage between catalepsy and the 5‐HT 1A receptor gene was verified using breeding experiment. Selective breeding for high predisposition to catalepsy was started from backcross BC[CBA × (CBA × AKR)] generation between catalepsy‐prone (CBA) and catalepsy‐resistant (AKR) mouse strains. CBA and AKR strains also differed in the 5‐HT 1A receptor functional activity. A rapid increase of cataleptic percentage from 21.2% in the backcrosses to 71% in the third generation of selective breeding (S 3 ) was shown. The fragment of chromosome 13 including the 5‐HT 1A receptor gene was marked with D13Mit76 microsatellite. Breeding for catalepsy increased the concentration of CBA‐derived and decreased the concentration of AKR‐derived alleles of microsatellite D13Mit76 in the S 1 and S 2 . All mice of the S 9 and S 12 were homozygous for CBA‐derived allele of D13Mit76 marker. Mice of the S 12 showed CBA‐like receptor activity. These findings indicate that selective breeding for behavior can involve selection of polymorphic variants of the 5‐HT 1A receptor gene.