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Responses of periodontal ligament stem cells on various titanium surfaces
Author(s) -
Heo YY,
Um S,
Kim SK,
Park JM,
Seo BM
Publication year - 2011
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2010.01728.x
Subject(s) - periodontal ligament stem cells , osteocalcin , periodontal fiber , osteopontin , osseointegration , titanium , chemistry , dental alveolus , dentistry , materials science , alkaline phosphatase , implant , biology , medicine , surgery , endocrinology , biochemistry , organic chemistry , enzyme
Oral Diseases (2011) 17 , 320–327 Objective: Periodontal ligament has been reported to have adult stem cells (PDLSCs) which are responsible to regenerate the alveolar bone tissue after tooth is removed from its socket. Also PDLSCs may be the stem cells responsible for the osseointegration of titanium implants after installing the implant immediately in the fresh extracted socket. Here we tested cellular responses of PDLSCs on the various titanium surfaces to verify this notion. Materials and methods: Titanium disc were prepared for the different surface textures; smooth machined, blasted with 75 and 125 μm Al 2 O 3 particles, and anodized. PDLSCs were cultured on these titanium discs and tested their proliferation and gene expressions of osteocalcin, osteopontin, type I collagen, and GAPDH. Results: Proliferation of PDLSCs was higher on the rough surface blasted with 75 μm Al 2 O 3 particles. Osteocalcin expression was increased on the Al 2 O 3 particle treated‐surface regardless of its particle size. Type I collagen expression was generally decreased with time in 6 days culture. Conclusions: In this experiment, it was shown that cultured PDLSCs proliferate in higher rate on the rough surface especially at the 75 μm Al 2 O 3 particle treated surface than other surfaces. Also, osteocalcin was highly expressed on the rough surfaces treated with 75 μm and 125 μm Al 2 O 3 particles.