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Bioactivity‐guided separation of anti‐acidogenic substances against Streptococcus mutans UA 159 from Polygonum cuspidatum
Author(s) -
Kwon YR,
Son KJ,
Pandit S,
Kim JE,
Chang KW,
Jeon JG
Publication year - 2010
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2009.01636.x
Subject(s) - streptococcus mutans , chemistry , chromatography , acidogenesis , bacteria , biology , organic chemistry , genetics , methane , anaerobic digestion
Oral Diseases (2010) 16 , 204–209 Objectives: The aim of this study was to separate the anti‐acidogenic substances against Streptococcus mutans UA 159 from Polygonum cuspidatum . Material and methods: The anti‐acidogenic substances were separated by a series of liquid–liquid fractionations followed by normal‐phase silica gel liquid chromatography, based on high‐performance liquid chromatography and glycolytic pH‐drop assay. The effectiveness of the separated substances on the acidogenicity of Streptococcus mutans UA 159 was examined using sodium fluoride as a positive control. The chemical composition and quantities of the components of the substances was also assessed by qualitative–quantitative chromatographic analysis. Results: Among the substances separated from P. cuspidatum , F3 showed the strongest inhibitory effect on the acidogenicity of S. mutans UA 159 in a dose‐dependent manner without displaying any bactericidal activity. F3 decreased the acidogenicity of S. mutans even at 12.5 μg ml −1 ( P < 0.05). F3 consisted mainly of resveratrol and emodin (C 14 H 12 O 3 and C 14 H 4 O 2 (OH) 3 CH 3 , respectively), which made up approximately 60% of the weight of F3. Conclusion: F3 can be considered as a promising agent for controlling the acidogenicity of S. mutans and subsequent dental caries formation.