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Combination of β‐TCP and BMP‐2 gene‐modified bMSCs to heal critical size mandibular defects in rats
Author(s) -
Zhao J,
Hu J,
Wang S,
Sun X,
Xia L,
Zhang X,
Zhang Z,
Jiang X
Publication year - 2010
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2009.01602.x
Subject(s) - osteopontin , stromal cell , alkaline phosphatase , osteocalcin , bone healing , von kossa stain , chemistry , bone morphogenetic protein 2 , regeneration (biology) , bone morphogenetic protein , bone marrow , pathology , microbiology and biotechnology , anatomy , biology , medicine , in vitro , endocrinology , gene , biochemistry , enzyme
Objective: To investigate the effects of mandibular defects repaired by a tissue engineered bone complex with β‐tricalcium phosphate (β‐TCP) and bone morphogenic protein‐2 (BMP‐2) gene‐modified bone marrow stromal cells (bMSCs). Materials and methods: bMSCs derived from Fisher 344 rats were cultured and transduced with adenovirus AdBMP‐2, AdEGFP gene in vitro . Osteogenic differentiation of bMSCs was determined by alkaline phosphatase staining, von Kossa assay and reverse transcription‐polymerase chain reaction. Gene transduced or untransduced bMSCs were seeded on β‐TCP scaffolds to repair mandibular full thickness defects with a diameter of 5 mm. Eight weeks post‐operation, X‐ray examination, micro‐computerized tomography and histological and histomorphological analysis were used to evaluate the bone healing effects. Results: Alkaline phosphatase staining and mineralized nodules formation were more pronounced in AdBMP‐2 group 14 days after gene transduction when compared with that of AdEGFP or untransduced group. The mRNA expression of osteopontin and osteocalcin also significantly increased 9 days after AdBMP‐2 gene transduction. Mandibular defects were successfully repaired with AdBMP‐2‐transduced bMSCs/β‐TCP constructs. The percentage of new bone formation in AdBMP‐2 group was significantly higher than that of other control groups. Conclusions: Bone morphogenic protein‐2 regional gene therapy together with β‐TCP scaffold could be used to promote mandibular repairing and bone regeneration.