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Increased expression of integrin α2 and abnormal response to TGF‐β1 in hereditary gingival fibromatosis
Author(s) -
Zhou J,
Meng LY,
Ye XQ,
Von den Hoff JW,
Bian Z
Publication year - 2009
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2009.01567.x
Subject(s) - immunohistochemistry , integrin , transforming growth factor , flow cytometry , fibroblast , fibromatosis , pathology , medicine , chemistry , biology , cell culture , immunology , receptor , genetics
Objective: To investigate the possible correlation between integrin α1, α2, and β1 expression and excessive collagen synthesis in fibroblasts from 3 unrelated Chinese families with hereditary gingival fibromatosis (HGF). Design: Gingival fibroblasts from three Chinese HGF patients and three healthy subjects were included. The expression of α1, α2, and β1 integrin subunits was examined by immunohistochemistry, quantitative PCR, and flow cytometry. We also investigated the effects of transforming growth factor‐β1 (TGF‐β1) on the expression of these integrin subunits. Results: Our results demonstrate that the expression of α2 was significantly higher in HGF fibroblasts compared with control fibroblasts ( P < 0.01). No significant differences in the expression of α1 and β1 were detected. Furthermore, TGF‐β1 promoted the expression of α1 and α2 in fibroblasts from both HGF patients and controls. However, it had a larger effect on the expression of α2 in HGF fibroblasts than in control cells. In contrast, α1 expression was stimulated more in control fibroblasts. Conclusion: The increased expression of integrin α2 and the increased response to TGF‐β1 of HGF fibroblasts may be related to the excessive collagen deposition in HGF patients.