Premium
TNF‐α and IL‐4 levels in generalized aggressive periodontitis subjects
Author(s) -
Bastos MF,
Lima JA,
Vieira PM,
Mestnik MJ,
Faveri M,
Duarte PM
Publication year - 2009
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2008.01491.x
Subject(s) - bleeding on probing , tumor necrosis factor alpha , aggressive periodontitis , medicine , gastroenterology , interleukin , periodontitis , tumor necrosis factor α , immunology , cytokine
Objectives: The aim of this study was to evaluate tumor necrosis factor (TNF)‐α and interleukin (IL)‐4 levels in healthy sites and sites exhibiting signs of moderate and advanced generalized aggressive periodontitis (GAgP) in the same subject. Methods: The following sites were selected for crevicular fluid sampling in the same AgP subject ( n = 14): Healthy sites (HS): no marginal bleeding or bleeding on probing (BOP) and probing depth (PD) ≤ 3 mm; Moderate sites (MS): BOP and PD between 4 and 6 mm; Advanced sites (AS): BOP and PD ≥ 7 mm. One site from periodontally healthy subjects ( n = 13) was sampled for use as a control. TNF‐α and IL‐4 levels were measured using ELISA. Results: The total amount of TNF‐α was lower for control sites, while there were no differences among healthy and diseased sites from GAgP subjects ( P < 0.05). The concentration of TNF‐α was higher in HS, in relation to the other sites ( P < 0.05). There were no significant differences among the groups regarding total amounts of IL‐4 ( P > 0.05), while IL‐4 concentration was significantly higher in control sites, when compared with sites from GAgP subjects ( P < 0.05). Conclusion: In conclusion, high levels of TNF‐α and low levels of IL‐4 were observed in both healthy and diseased sites within the same generalized AgP individuals.