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Expression of two drug‐metabolizing cytochrome P450‐enzymes in human salivary glands
Author(s) -
Kragelund C,
Hansen C,
Torpet LA,
Nauntofte B,
Brøsen K,
Pedersen AML,
Buchwald C,
Therkildsen MH,
Reibel J
Publication year - 2008
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2007.01415.x
Subject(s) - cyp1a2 , saliva , serous fluid , xenobiotic , salivary gland , oral mucosa , cyp3a4 , biology , cytochrome p450 , drug metabolism , ductal cells , immunohistochemistry , parotid gland , in situ hybridization , enzyme , medicine , endocrinology , pathology , messenger rna , immunology , biochemistry , anatomy , gene
Objective: The oral cavity is constantly lubricated by saliva and even small amounts of xenobiotics and / or their metabolites in the saliva may affect the oral mucosa. Our aim was therefore to clarify if xenobiotic metabolizing enzymes CYP1A2 and CYP3A4 are expressed in salivary glands. Methods: Formalin‐fixed paraffin‐embedded specimens from parotid (10), submandibular (7) and labial (10) salivary glands were examined immunohistochemically and by in situ hybridization for expression of CYP1A2 and CYP3A4 protein and mRNA. Results: CYP1A2 and CYP3A4 protein and mRNA were detected in ductal and seromucous / serous acinar cells in all gland types although to a varying degree and intensity. Mucous acinar cells were positive to a lesser extent. Conclusion: The results indicate a xenobiotic metabolizing capability of salivary glands. This may have implications for development of oral mucosal disease as a result of mucosal exposure to metabolites originating from internal sources (blood) as well as from saliva.