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Effects of fluorides on Candida albicans
Author(s) -
Flisfisch S,
Meyer J,
Meurman JH,
Waltimo T
Publication year - 2008
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2007.01385.x
Subject(s) - buccal administration , candida albicans , chlorhexidine , sodium fluoride , chemistry , fluoride , corpus albicans , microbiology and biotechnology , buccal swab , phosphate , cell , saline , nuclear chemistry , pharmacology , biology , biochemistry , dentistry , medicine , inorganic chemistry , endocrinology
Aims: To assess whether a short exposure of Candida albicans to commonly used fluorides would affect growth, cell surface hydrophobicity, and adherence to buccal epithelial cells. Methods: Candida albicans ATCC 90028 and 11 clinical isolates were used. Minimal inhibitory concentrations (MICs) of sodium fluoride (NaF) and of an amine fluoride / stannous fluoride combination (AmF / SnF 2 ) were determined. Yeasts were exposed to MICs of tested agents for 1 h. Subsequently, their growth was recorded spectrophotometrically. Their cell surface hydrophobicity was assessed with n ‐hexadecane. Adherence to buccal epithelial cells was determined microscopically. Phosphate buffered saline (PBS) and chlorhexidine digluconate (CHX) served as controls. All results were analyzed by one‐way ANOVA. Results: MICs of AmF / SnF 2 and CHX varied between 1 and 4 μ g ml −1 , whereas those of NaF were 15 000 μ g ml −1 . Statistically significant growth inhibition was detected after AmF / SnF 2 (OD 24 h ± SD 0.457 ± 0.059) and CHX (0.175 ± 0.065) in comparison with PBS (0.925 ± 0.087) and NaF (0.813 ± 0.081). All strains demonstrated uniform behavior. Only minor changes in cell surface hydrophobicity and adherence to buccal epithelial cells (BEC) were detected. Conclusion: Growth inhibition of AmF / SnF 2 was comparable with that of CHX whereas NaF had a weaker effect. Exposure to the fluorides did not seem to alter the cell surface hydrophobicity nor adherence to BEC.