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Molecular detection and identification of Synergistes phylotypes in primary endodontic infections
Author(s) -
Siqueira JF,
Rôças IN
Publication year - 2007
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2006.01301.x
Subject(s) - phylotype , biology , polymerase chain reaction , amplicon , 16s ribosomal rna , microbiology and biotechnology , bacteria , genomic dna , gene , genetics
Aim: Uncultivated phylotypes from the Synergistes group have recently emerged as suspected endodontic pathogens. This study aimed to investigate the presence and identity of these bacteria in primary endodontic infections using a 16S rRNA gene‐based group‐specific heminested polymerase chain reaction (PCR) protocol. Materials and methods: Genomic DNA was isolated directly from clinical samples and used as templates for PCR. Amplicons from positive specimens were sequenced and phylogenetically analyzed to determine species identity. Results: Overall, about one‐third of the samples harbored Synergistes bacteria. The following phylotypes were disclosed: oral clones W028, BA121/P4G_18 P1, W090, BH017 and E3_33. Conclusions: These results demonstrated that as‐yet uncultivated Synergistes phylotypes are present in the endodontic microbiota and a role in causation of apical periodontitis is suggested though remains unproven.