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Saliva of patients with Type 1 diabetes: effect of smoking on activity of lysosomal exoglycosidases
Author(s) -
Knas M,
Karaszewska K,
Szajda SD,
Zarzycki W,
Dudzik D,
Zwierz K
Publication year - 2006
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2005.01190.x
Subject(s) - saliva , isozyme , diabetes mellitus , type 1 diabetes , endocrinology , medicine , type 2 diabetes , chemistry , biochemistry , enzyme
Objective: The aim of our study was to evaluate the influence of smoking on the activity of N ‐acetyl‐ β ‐hexosaminidase (HEX), its isoenzymes A (HEX‐A) and B (HEX‐B) and β ‐galactosidase (GAL), in the saliva of patients with Type 1 diabetes. Methods: In the supernatant HEX and its isoenzymes A and B, and β ‐galactosidase were determined by the method of Chatteriee et al in modification of Zwierz et al (mKat kg −1 of protein). Protein was determined by the Lowry et al method (mg ml −1 ). Results: The results presented here suggest that diabetes and smoking modify activity of HEX and its isoenzymes, but only combination of diabetes and smoking give a significant increase in the specific activity of HEX and its isoenzymes. Conclusions: Type 1 diabetes slightly changes the composition of saliva. Smoking cigarettes significantly modifies the composition and properties of saliva in healthy individuals and patients with Type 1 diabetes.