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The relationship between the relative amount of Porphyromonas gingivalis in saliva and halitosis
Author(s) -
Kato H,
Awano S,
Yoshida A,
Ansai T,
Takehara T
Publication year - 2005
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2005.01105_54.x
Subject(s) - saliva , porphyromonas gingivalis , dentistry , taqman , chemistry , fusobacterium nucleatum , bacteria , microbiology and biotechnology , medicine , real time polymerase chain reaction , periodontitis , biology , biochemistry , gene , genetics
Objectives Porphyromonas gingivalis is one of the microorganisms that most actively produce CH 3 SH and we have reported that subjects with P . gingivalis have higher CH 3 SH levels than subjects without P . gingivalis . However, little is known about the relationship between P . gingivalis levels in saliva and the condition of oral malodor. In this study, we evaluatedthe association between the relative amount of P . gingivalis in saliva and halitosis in mouth air. Methods All of the subjects were patients at the Preventive Dentistry and Breath Odor Clinic of Kyushu Dental College, where they received a periodontal examination. Volatile sulfur compounds (VSC: hydrogen sulfide and methyl mercaptan) were measured using gas chromatography. Saliva samples were collected in a sterile plastic tube over a period of 5 min while the subject chewed on paraffin wax, and were then immediately stored at –80°C until use. Template DNA was obtained from the stored saliva using an Easy‐DNA Kit (Invitrogen, CA, USA) according to the manufacturer's instructions. Conventional PCR assays were used to confirm the presence of P . gingivalis . A 5’ nuclease TaqMan PCR was used to quantify P . gingivalis in saliva. The relative numbers of bacteria were measured using the comparative threshold cycle method. Results We found a quantitative relationship between the P . gingivalis levels in saliva and the condition of halitosis in mouth air. Conclusion We analyzed the relationship between the relative amount of P . gingivalis in saliva and oral malodor.